Galaxy Training for Pathogen Genomics, AMR Detection & Virus ID


Welcome and introduction

Agenda, resources and other useful information

Galaxy at a Glance

A brief introduction to Galaxy. What is Galaxy? Why should you use Galaxy? How do you use Galaxy?

Introduction to Galaxy Analyses

How to get started in Galaxy. Here we'll cover the core tasks in Galaxy: uploading files, using tools, viewing histories, and running workflows.

Exploring datasets with statistics and scatterplots

Often, one or more data pre-processing step(s) may be required to proceed with the analysis. And, after the analysis, maybe you would like to visualize the result or datasets

Quality Control

Introduction to quality control of NGS raw data: key quality metrics, common issues, and strategies to improve dataset quality.

Genome Assembly of a bacterial genome (MRSA)

Bacterial genome assembly from Illumina MiSeq reads, covering data quality assessment, assembly generation, and assembly quality metrics.

Bacterial Genome Annotation

Annotation and exploration of a draft bacterial genome, including gene prediction, identification of genomic components, evaluation of annotation results, and visualization of annotated features.

Identification of AMR genes in an bacterial genome

Identification and visualization of antimicrobial resistance genes in a bacterial genome, including their genomic location and association with plasmids.

Pathogen detection with ABRicate

Identification of pathogens from sequencing data and tracking their presence across multiple samples.

Pangenome Analysis

If we analyse DNA from several bacterial strains, we may want to know which genes they have in common and which are unique to some strains.

Microbial Variant Calling

How do we detect differences between a set of reads from a microorganism and a reference genome?

Extras

Optional resources and tutorials to help you get more confident with Galaxy and its everyday usage.

Hands On

From raw reads to insights: A hands-on guide to bacterial genomics


Authors and Contributors

Some of the materials used in this training are from the Galaxy community and are the result of a collaborative work. Thanks to all the contributors and the Galaxy Training Network!